Coding

Part:BBa_K1941000:Design

Designed by: Dimitri Coukos   Group: iGEM16_EPFL   (2016-10-13)


PCP fused to Mxi1 domain


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 77
    Illegal BglII site found at 333
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Mxi1 was fused to PCP based on the fusion between VP64, a transcriptional activator, and MCP, another RNA binding module described in "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015. The link between PCP and Mxi1 is 6 base pairs long.

Source

PCP sequence taken from "Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds", J.G. Zalatan, Cell 2015

References